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SCIENTISTS:
1.
Dr. Satadal Chakraborty, Scientist-C
MANDATE:
1. To
evolve integrated silkworm disease management strategies for the
prevention/ control of silkworm diseases
2. To
act as a nodal centre for co-ordinating silkworm disease survey
and surveillance programme in the eastern and north-eastern
parts of India
3. To
extend R&D support to the sericulturists in the eastern and
north-eastern parts of India
ACHIEVEMENTS:
Ø LABEX a
general silkworm bed-disinfectant, developed by this section,
has been patented (Patent
No. 200199) and
commercialized.
Ø A room
disinfectant, 5%
Bleaching powder solution has
been developed for the prevention of various silkworm diseases
Ø Developed
a new general silkworm bed-disinfectant, SERICILLIN specifically
for control of muscardine disease.
Ø Gattine
disease is controlled by using 1% chlorine (bleaching powder
solution) as room disinfectant and feeding of 700ppm
Streptomycin sprayed leaves including dusting of Labex.
Ø Identified
two botanicals for control of muscardine and grasserie diseases
Ø Elucidated
the structure and nature of haemocytes of Bombyx
mori L. and their
role in the cellular defensive response.
Ø Identified
disease tolerant silkworm races from the germplasm based on
haemocyte immunity study
Ø Developed
a simple agglutination test for early detection of BmNPV
infection in B.
mori L.
Ø An
Agar Gel Double Diffusion Assay has been standardized for
distinguishing the various serotypes of Bm NPV
Ø Identified
hotspots for various disease occurrences in West
Bengal and
developed a forewarning calendar of silkworm disease incidence.
RESOURCES:
Ø Autoclave
Ø Centrifuge(REMI)
Ø Cyclomixer
Ø CO2 Incubator
Ø Leitz
Diaplan compound microscope, Germany.
Ø Laminar
Flow
Ø Water
Purification System (Sartorius)
Ø Cell
Sonicator
ONGOING PROJECTS / PILOT STUDY:
ARP3351:
Studies on the morphology, life cycle and pathogenicity of some
microsporidia infecting silkworm, Bombyx
mori L. and their
identification
Objectives:
a. To
study the biology of microsporidia
b. To
identify the virulent microsporidia based on their pathogenicity
to Bombyx mori L.
c. To
investigate the impact of microsporidian infection on growth and
development ofBombyx mori L.
d. To
identify the molecular markers for the identification of
virulent and non-virulent microsporidia.
PILOT STUDY:
Immunization of silkworm, Bombyx mori L. against bacterial
disesase
Objectives:
a. Immunization
of disease tolerant silkworm breeds identified so far.
b. The
resultant protein rich compound / heat killed bacteria /other
preferably low cost chemicals/botanicals suitable for
immunization may be patented for the benefit of Sericulture
industry.
CONCLUDED PROJECTS:
ARP3285: Studies
on the pathogens, pathogenesis and control of gattine in
silkworm Bombyx
mori L
Outcome: It
was found that Gattine disease is caused by an ultra virus and a
bacterium (Staphylococcus vitulinus). Gattine disease is
controlled by using 1% chlorine (bleaching powder solution) as
room disinfectant and feeding of 700ppm Streptomycin including
dusting of Labex as per recommendation, without affecting the
economic characters of the cocoons.
ARP3286: Studies
on the prevention and control of Nuclear Polyhedrosis and White
Muscardine diseases of silkworm using botanicals
Outcome: Identified
an eco-friendly, effective and economically viable two botanicals
for the prevention/ control of Grasserie and White
Muscardine diseases
of silkworm, Bombyx
mori L.
Spray of 5% aqueous crude extract of PE-02 after every moult
(30-45 m before resumption of feed) and 4th day
of 5th instar
is found to be very effective against White
Muscardine
Spray of 7% aqueous
crude extracts of PE-10 is used as a preventive/ control measure
against Grasserie disease
by per oral administration
through mulberry leaves after each moult (30-45
m before resumption
of feed) and on 4th day
of 5th instar
ARP004: Studies
on immunity and immunodiagnosis of silkworm diseases
Outcome: A
simple agglutination test has been developed for detection of
BmNPV infection. An agar gel double diffusion assay has been
standardized for distinguishing the various serotypes of BmNPV
ARP008:
Haemocyte immunity study of silkworm, Bombyx
mori L.
Outcome: On
the basis of cellular defense response to bacterial/ particulate
virus infection, five multivoltine [Nistari, Debra, Nistari (DN)P,
M6DP & M12(W)] and four bivoltine (SK3, SK6, BHR1 and BHR2) were
identified as relatively tolerant from the germplasm
ARP002: Physiotherapeutic
and chemotherapeutic studies towards control of silkworm
diseases
Outcome: Recommended
hot HCl treatment (1.05 sp. gravity at 46ºC for 30 m) for
controlling pebrine at primary infection and Albendazole (0.5%)
as feed to control pebrine at secondary infection.
Nystatin
(0.4%) was identified and recommended to control White
Muscardine disease
ARP003:
Studies on the improvement of disinfection
Labex,
a silkworm bed-disinfectant and 5%
Bleaching powder solution, an effective room disinfectant
were formulated and recommended for the prevention of various
silkworm diseases
ARP005:
Survey and surveillance of silkworm diseases and its management
inWest Bengal
The pattern
of incidence and hotspots for various silkworm diseases in
commercial crop in West
Bengal were
identified
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