CSR&TI, BERHAMPORE, WEST BENGAL
HIGHLIGHTS OF CONCLUDED RESEARCH PROJECTS WITH REFERENCE TO ACHIEVEMENT OF THE OBJECTIVES, OUTPUT AND UTILITY

(As on 09.12.2020)

Sl. No.

Project Code, Title & period

Duration / Project Period

Objectives

Achievement / Output

Utilization status of the output

I. SILKWORM IMPROVEMENT
1

AIP 3421: Physio-biochemical characterization of selected silkworm germplasm accessions during seed crop seasons

April 2008 - March 2011

  1. Physio-biochemical characterization of silkworm germplasm accessions for quality assessment and identification of physio- biochemical markers, if any,
  1. Silk conversion was higher in M6M81 & MCon1 and high fecundity in M6DPC.

  2. During adverse season (Sept), M.Con.4 showed superior survival and M6M81 in silk conversion.

  3. SK4C (BV) was superior in survival, growth rate, critical weight & silk conversion.

  1. Information generated was used to evaluate silkworm hybrids/breeds in breeding programme
2

AIB 3393:  Development of commercial hybrids of silkworm Bombyx mori L. by identification of bivoltine parents through screening in different seasons of West Bengal

Dec 2006 - April 2011

  1. Identification of suitable bivoltine breeds & foundation crosses for the seed crop rearing in favourable and unfavourable seasons
  1. Three Multi x Bi hybrids viz.,  M6DPC x SK4C, M6DPC x (D6PN x SK4C) and M6DPC x D6PN were  found superior with 55-56kg/100 dfls against N x NB4D2 (48.3 kg)
  2. Bivoltine hybrid, D6PN x SK4C recorded improved  cocoon yield (43.9 kg /100 dfls) over NB18 x P5 (38.3 kg) in multi- locational trials
  1. The improved hybrids/breeds were evaluated at the field level with better success
3

MSRAP:  Mulberry Silkworm Race Authorization Prog. (Phase-VIII) 

Jan 2011 - Dec 2012

  1. To identify region and season specific hybrids
  1. Multi x Bi hybrid: M6DPC x SK4C stood first followed by Nistari x (SK6 x SK7).
  2. Bi x Bi hybrid: The double hybrid (CSR50.52) x (CSR51.53) stood first followed by CSR50 x 51 and CSR28DR x 21DR
  1. M6DPC x SK4C & Nistari x (SK6 x SK7) were authorized during 2013-14 (XV HAC)
4

AIP 3472: Standardization and determination of temperature tolerance potentiality in different developmental stages of silkworm, Bombyx mori L.

Sept 2011 - Aug 2014

  1. Determination of stage specific effect of temperature (30-35°C) and humidity (>90%) on silkworm rearing.
  2. Determination of temperature and humidity tolerance on 50% lethality and period of exposure
  1. Bivoltine breed, BCon4 and multivoltine breed, MCon4 were found as productive & thermo-tolerant as compared to Nistari.
  2. NB4D2 was found most susceptible among the bivoltines.
  3. Sarupat was also found severely affected with thermal stress > 32 ºC.
  1. Identified GRs were utilized to develop thermo-tolerant breeds through MAS
5

AIE 3454: Evaluation of elite bivoltine silkworm germplasm under different agro climatic conditions: All India Silkworm Germplasm Evaluation Programme
[Phase-II project of  CSGRC, Hosur]

Sept 2011 - Aug 2014

  1. Identify suitability of bivoltine silkworm germplasm for specific agro climate
  2. Identify the potential germplasm as parents for silkworm hybridization
  1. Silkworm accessions BBE-268 (60), BBI-348 (57.8) and BBI-338 (56.9) showed higher evaluation index and could be the potential parents in  silkworm improvement programmes
  1. Identified silkworm accessions were utilized in  silkworm improvement programmes
6

AIB 3491: Post Authorization trials of silkworm hybrids in Eastern & North-Eastern India

Sept 2012 - Dec 2014

  1. To popularize the authorized hybrids to the farmers of Eastern and NE India
  1. Multi x bi hybrids, MCon1 x BCon4 and MCon4 x BCon4 were recommended for favourable seasons
  2. Multivoltine hybrids, Nistari x MCon4 and MCon1 x MCon4 were recommended for unfavourable seasons of West Bengal and Jharkhand states.
  3. Bivoltine hybrids, Gen3 x Gen2 in West Bengal & Jharkhand, while FC1 x FC2 in North Eastern states were recommended
  1. MCon1 x BCon4, MCon4 x BCon4 &  MCon1 x MCon4 were utilized for commercial exploitation in West Bengal, Jharkhand & other E & NE states
7

APS 3497: Studies on the environmental effect on P1 rearing, its’ grainage performance followed by commercial rearing of silkworm, Bombyx mori L., during unfavorable seasons of West Bengal

May 2013 - Apr 2015

  1. Determination of the effect of  environmental factors on P1 seed crop rearing during adverse crop seasons and its effects on  commercial grainage performance
  1. Environmental factors such as temperature & RH influences performances of silkworm seed crop rearing, reproductive potential & subsequent commercial grainage with the popular hybrid.
  1. Information generated was shared with NSSO/RSPs for addressing the grainage issues especially during unfavourable crop seasons in West Bengal
8

AIB 3496: Development of high temperature and high humidity tolerant bivoltine breeds of silkworm (Bombyx mori L.)

July 2012 - Jun 2015

  1. To develop a method of induction for thermal stress In silkworm.
  2. To develop  high temperature and high humidity tolerant breed
  1. Ten HTH breeds were short listed under high temperature (35±1°C) and high humidity (85±5%) conditions
  1. Few identified GRs were utilized to develop breeds tolerant to thermo & RH in ongoing project (AIB-3602; WB series) through MAS
9

AIB 3531: Authorization trials of silkworm hybrids in Eastern and North-Eastern India

Aug 2014 - July 2015

  1. To evaluate the newly developed hybrids at farmers
  2. To identify the suitable hybrids for commercial exploitation
  1. Improved cross breed, M6DPC x (SK6.7) and bivoltine hybrid, BCon1 x BCon4 were found superior & recommended for E & NE India states
  1. M6DPC x (SK6.7) and BCon1 x BCon4 were authorized during 2017-18
10

AIB 3501: Development of multivoltine breeds of silkworm (Bombyx mori L.) with high shell percentage and high neatness of silk filament

July 2013 – June 2016

  1. To develop multivoltine breed with high cocoon shell %  (16-18%) and high neatness (80-85 Points) of silk filament to suit the climatic conditions of West Bengal
  1. Utilizing MH1, Gen3, Mcon1 & MCon4 multivoltine lines viz., 12Y, 21 Y & 8W with 17-18% SR & >80 points neatness were developed.
  1. Shortlisted breeds/hybrids were evaluated through On farm trail AIB-3616 (2017-2020)
  2. 12YxBV was superior over M6DPC/Nistari hybrids through OFT & 12YXBFC1 is recommended for  authorization trial
11

AIB 3480: Development of silkworm Bombyx mori L. breeds from a gene pool with higher genetic plasticity

Sept 2012 - Aug 2016

  1. To find out the genetic Plasticity for the target traits among bivoltine & multivoltine breeds by raising converged gene pool
  1. Using congenic approach, promising 6-way crosses of Multivoltine/Bivoltines were developed with improved economic traits & survival
  1. GRs are utilized to develop RBL lines in the project AIB-3619 (2017-2020) through six-way crosses
12

AIB 3466 : Development of region specific bivoltine breeds suitable for highly fluctuating and seasonally variable climatic conditions of   E & NE India

 Aug 2011 - Dec 2016

  1. To develop bivoltine breeds with genetic plasticity to buffer against adverse climatic conditions of West Bengal
  1. Through shuttle breeding approach, ten new bivoltine breeds (BHP-1, BHP-2, BHP-3, BHP-4, BHP-5, BHP-6, BHP-7, BHP-8, BHP-9 and BHP-10) were developed.
  2. New bivoltine hybrid combinations to be identified with better genetic plasticity
  1. Utilizing BHP breeds, a bivoltine double hybrid (BHP3.2 x BHP8.9) was developed in the project (AIB 3617; 2017-2020)
13

AIB 3514: Development of multivoltine based congenic / NIL breed of silkworm (Bombyx mori L.) through introgression of “Id” character and its’ use

June 2014 -  May 2017

  1. Development of Multivoltine (V3) based congenic/ NIL (Near Isogenic Line) / breed through introgression of target Id (Inhibiter of diapause) character
  1. Four oval and one dumbbell breeds viz., M.Con.4Id (Pseudo-p), M.Con.4Id (Non- pseudo-p), M.Con.4Id (Pseudo-p), B.Con.4 Id (Pseudo-p) and BHBId (Pseudo-p) with Id character have been developed.
  1. Id breeds developed have hatching issues, especially in bi x multiId                         (< 90%)
14

AIB 3547: Development of high temperature and high humidity tolerant bivoltine breeds of silkworm

July 2015 - June 2017

  1. To develop thermo tolerant bivoltine breeds with genetic plasticity to buffer against the adverse climatic conditions of West Bengal
  1. Two new hybrids viz., HTH3×HTH6 & HTH4×HTH9 were found tolerant to high temp. & high humidity (±1°C & 85±5%).
  2. The breed performance was on-par with controls (tolerance & productivity).
  1. HTH lines are maintained as breeding resource material in germplasm
15

AIB 3545: Authorization Trial of Silkworm hybrids in Eastern and North Eastern India

Aug 2015 - March 2018

  1. To test the hybrids with the farmers of Eastern and North-Eastern India for its authorization 
  1. New Bi x Bi hybrid (BCon1 x 4) and Multi x Bi hybrid (M6DPC x (SK6.7) were evaluated (2014-2017) in West Bengal, Jharkhand, Odisha & NE states.
  2. M6DPC x (SK6.7) & BCon1 x 4 performed better than existing hybrids in E & NE.
  1. M6DPC x (SK6.7) & BCon1 x 4 have been authorized for Eastern and North-Eastern states by HAC.
16

AIB 3614: Evaluation and identification of suitable bivoltine hybrids for Odisha

Oct 2017 - Nov 2019

  1. To identify bivoltine hybrids with better productivity traits suitable for Odisha
  1. Among 12 silkworm hybrids, FC1xFC2 performed better with 21.27% shell across the trials.
  1. FC1 x FC2 is being popularized in Odisha through DoS programmes
17

AIB 3616:  On farm trial of the multivoltine silkworm breeds/ hybrids developed for high shell percentage and neatness of silk filament

Sept 2017 - Dec 2019
  1. To test the potentiality of new improved MV breeds with high shell% and neatness (AIB 3501).
  2. To identify season specific silkworm hybrids for West Bengal, Jharkhand Odisha and Assam
  1. Around 3000 dfls of each hybrid combination was tested in different states during the OFT. 12(Y) x BFC1 performed better followed by21(Y) x (BCon1.4) & 8(W) x (SK6.7).
  2. 12(Y) recorded an avg. cocoon yield of ~52kg with ~10% improvement over N x SK6.7 authorization trial
  1. Authorization trials of silkworm hybrid, 12YxBFC1 in E&NE states with project code No. AIB02009MI (2020-22)
18

AIB 3617 Identification of region specific bivoltine hybrids suitable for highly fluctuating and seasonally variable climatic conditions of E & NE India 

April 2017- March 2020

  1. To identify bivoltine hybrids with genetic plasticity to buffer against the adverse climatic conditions of Eastern and North-Eastern India
  1. Developed a bivoltine double hybrid, (BHP3.2 x BHP8.9) with an improved shell (10-12%) over SK6 x SK7 & Bcon1 x Bcon4 (avg. yield: ~65kg)
  1. The double hybrid BHP 3.2 x BHP 8.9 is being evaluated under OFT (2020-21) in E & NE region 
II. HOST PLANT IMPROVEMENT
1.

PIB 3422: Development of mulberry genotypes for improvement in productivity and quality

Oct 2008 - March 2012

  1. To develop high yielding mulberry genotypes suitable for silkworms
  1. Seven genotypes (C-01, C-02, C-09, C-11, C-05, C-52 & C-47) recorded higher leaf yield with 19.5-58.5% improvement over the check (S-1635).
  1. Identified genotypes are utilized for further evaluation under Final Yield Trial (FYT) in new project.
2

PPS 3435: Studies on micronutrients for sustained high productivity of quality mulberry in Eastern and North-Eastern India. (Collaboration with 4 RSRSs)

Jan 2010 - June 2013

  1. To evaluate the micro-nutrients’ status of mulberry growing soils
  2. To work out the recommendation for individual micronutrient through MLTs
  3. Micronutrient requirement by under appropriate statistical package of DRIS
  1. Optimum foliar requirement of micronutrient for mulberry in E & NE India using DRIS
  2. Zn in WB plains, Orissa, Jharkhand & NE states; Mn in WB hills & Cu in NE states were diagnosed as critical.
  3. Optimum requirement of foliar Zn has been computed 16.56, 12.53 and 19.02 mg/Kg for WB plains, Orissa, Jharkhand and NE.
  4. Optimum requirement of foliar Mn has been computed to 40.73 mg/Kg for WB hills and Cu of 5.01 mg/Kg for North-Eastern states.
  5. Critical value for Zn has been computed 0.73, 0.48 and 0.44 mg/Kg for WB plains, Orissa as well as Jharkhand & NE states
  6. Critical value for Mn (2.69 mg/Kg) for WB hills and Cu (0.45 mg/Kg) for NE states.
  1. Recommendation for foliar spray of Zn (0.2%; twice in a crop) & Mn (0.1%) for WB plains, Orissa and Jharkhand & NE states
  2. Spray would be scheduled in terms of critical value of soil availability for individual micronutrients
3

PPF 3487: DSS initiative through impact assessment of agro-climate on foliage yield of mulberry

Oct 2012 - Sept 2013

  1. Spatio-temporal historical data mining on agro climate and mulberry foliage yield, data processing and analysing
  1. A synthetic mulberry yield weather model [Foliage yield = 3.211 – 0.153 x crop + 0.003 x hit stress load (mega Jule/second – 0.03 x solar radiations – 0.101 x max. temp. (°C)] was developed using historical crop yield and corresponding spatio-temporal weather data from 2002 to 2012
  1. Best fit robust models were developed in the GDD project for mulberry yield estimations
4

PIP 3469: Screening of early sprouters and late senescence mulberry variety with better leaf yield and quality under low temperature conditions

Nov 2011 - Oct 2014

  1. Screening of mulberry accessions  having early sprouting and late senescence characters with better leaf yield and quality under low temperature condition
  1. Identified early sprouting, late senescence high yielding accessions with better quality
  2. 149 mulberry accessions (25 exotic, 51 indigenous, 38 C-lines and 35 S lines) were screened.
  3. Accessions C-1540 and C-1726 recorded maximum leaf yield with moderate quality
  1. C-1540 and C-1726 were recommend for breeding programme as one of the parents developing high yielding cold tolerant mulberry
5

PPS 3452: Terrestrial carbon sequestration for sustained high productivity of quality mulberry

Jul 2011 - June 2015

  1. To enumerate the enhanced organic carbon stock of the soil due to the induction of altered farming practices in mulberry dim fit to carbon sequestration with comparison to existing one
  1. Mulberry growing with moderate tillage + grass cover registered highest leaf productivity (38.72 t/ha/year) as compared to existing farming practice (intensive tillage; 38.16 t/ha/year).
  2. In terms of CSP also, moderate tillage with grass cover registered highest value of 6.90 t/ha/year followed by existing farming practice (intensive tillage; 6.54 t/ha/year).
  3. Estimated soil organic carbon stock (SOCS) under moderate tillage with grass cover was 40.16 Mg/ha; while under existing farming practice was 35.25 Mg/ha.
  1. Carbon sequestration data was documented through MLTs
6

PIB 3424: Development of low temperature stress tolerant mulberry genotypes for sub-tropical plains

Jan 2009 - Dec 2015

  1. Development of low temperature stress tolerant mulberry genotypes capable of providing higher leaf yield during Aghrahyani & Falguni silkworm rearing seasons than the ruling variety S-1635
  1. Identified fifteen progenies with early sprouting, EC, NR activity, leaf yield etc., during winter seasons under PRT.
  2. Identified seven mulberry genotypes i.e., C-29,C-33,C-45,C-108,C-212,C-225 and C-384 with higher growth, biochemical parameters,  moulting test and leaf yield over the check S-1635 through Primary Yield Trial
  1. Identified four promising genotypes i.e., C-45, C-108, C-212 & C-384 with              >30% leaf yield over check S-1635
  2. Utilized under the project PIB 3576 (2016-2020) for commercial use
7

PIB 3479: Development of high yielding mulberry varieties using physiological growth parameters

Oct 2012 - Sept 2016

  1. To develop mulberry varieties with superior quality and with 10% higher leaf yield over the ruling variety
  1. Developed 1024 hybrid seedlings from six female & eleven male mulberry germplasm with better physiological traits and leaf yield.
  2. Identified 24 genotypes with significantly higher leaf yield (2.104 to 2.561 kg/plant/y) against S-1635 (1.620 kg/plant/y)
  1. Identified  24 promising genotypes were evaluated further under Primary Yield Trial in PIB-3610
8

AICEM-III: All India Coordinated Experimental Trail for Mulberry (AICEM) - Phase III, (A prog. of C.O., Bangalore

Aug 2011 - Dec 2016

  1. To identify and authorize suitable mulberry variety for commercial use in different agro-climatic mulberry cultivation zones
  1. Identified high yielding mulberry genotype, C-2038 with average annual leaf yield of 53.8 t /ha/yr in irrigated and 17.3-21.3 in rainfed, which is >20% higher over S-1635.
  2. The genotype Tr-23(11-12 t/ha/y) in hilly showed 42% higher leaf yield potential than zonal check, S-146
  1. C-2038 & Tr-23 were authorized for commercial cultivation for farmer in E & NE India during 2017-18
9

PPF 3532: Assessment, Development and Management of area under mulberry in major sericulture districts of West Bengal

Feb 2015 - Jan 2017

  1. To estimate the current spatial extent of mulberry cultivation WB using Remote Sensing, GIS and Global Positioning Systems
  2. To estimate leaf protein and moisture contents using hyperspectral data
  3. To develop block specific MIS for SILKS portal for dynamic visualization
  1. Maximum area of west Bengal covered by S1 & local as compared S-1635
  2. MIS for Mulberry growing districts of WB using Remote Sensing, GIS and Global was developed.
  3. Moisture & protein levels of mulberry foliage were discriminated by NDVI & OSAVI 1510.
  1. High resolution multispectral images (< 1m resolution) would be useful for identification of farmer’s mulberry fields
10

PIB 3515: Evaluation of new developed triploid mulberry varieties for productivity and quality

June 2014 - March 2017

  1. Evaluation of newly developed triploid mulberry varieties under irrigated condition for better productivity and quality
  1. Fifteen promising triploid genotypes (PIB-3365) along with check (S-1635) were evaluated for leaf yield, biochemical and disease & pest incidence.
  2. Identified seven genotypes (C-105, C-252, C-116, C-131, C-57, C-174 and C-124) with higher leaf yield (>15%) and biochemical values over S-1635; 319g/plant/crop).
  3. Disease and pest incidence was below ETL
  1. Utilizing these triploid genotypes in mulberry breeding projects for further commercial exploitation
11

APS 3539: Characterization of mulberry growing soils for nutrient management in Golaghat district of Assam. (Collaborative with NBSS&LUP, ICAR, Jorhat)

April 2015 - March 2017

  1. To characterize and classify mulberry growing soils and prepare data bank.
  2. To prepare soil test-based fertilizer recommendation
  3. To popularize the soil test-based fertilizer application
  1. Recommended 0.1 % foliar sprays of Boron along with recommended dose of fertilizers (RDF; 150:50:50) for micronutrient Boron deficit mulberry soils.
  2. Recommended 25 % extra of RDF for low fertile soils and 15% less of RDF for highly fertile soils
  1. Advisory to address the nutrient deficiency for Assam mulberry soils
12

PIN 3587: Improvement of leaf quality and productivity through external application of seaweed extracts in mulberry

Oct 2016 - Sept 2017

  1. To determine the effect of application of SWE on growth in mulberry biomass.
  2. Standardize dose- time intervals for application of SWE.
  3. To determine effect of SWE on foliar diseases.
  4. To perform bioassay with SWE.
  1. Application of Ascophyllum nodosum extract @ 0.5 ml /L at 21 and 28 DAP was suitable for increasing leaf yield (11%) in S-1635 variety as compared to control.
  1. SWE application improved leaf yield by 11% only.
  2. The techno-economic analysis of seaweed extract application seems to be less promising as compared to Morizyme.
13

PIB3521:  Assessment of promising powdery mildew resistance lines for perspective commercial use

Jan 2015 -
Dec 2017

  1. Evaluation of PM resistant promising lines for foliage biomass and associated traits.
  2. Bioassay for rearing efficiency.
  3. Development of segregating progeny from promising F1s.
  4. Assessment of PM resistance of developed progeny using MAS lines.
  1. C-1360 exhibited ~29% more leaf yield overS-1635.
  2. ~30 PM specific putative markers were tested on segregating progeny (3 generations) with phenotyping.
  3. Two allelic bands of 182 (MM68) and 190bps (MM128) showed strong correlation with phenotypic disease reaction.
  4. >80% correlation in F2s than F1 indicates refinement of trait over generations suggesting possibility of association of trait with the markers.
  1. Promising line (C-1360) is undertaken in AICEM phase IV (2018-2023)
14 

PIB 3481: Evaluation of mulberry varieties suitable for low in-put soil

Jan 2013 - Dec 2017

  1. To evaluate newly evolved promising mulberry varieties suitable for low input soil.
  1. Genotype, C-9 recorded highest leaf yield (49.89 & 34.01 t/ha/yr), which is 13% & 27% higher over S-1635 under full and half-NPK doses, respectively.
  2. C-9 bioassay was at par with Check, S-1635.
  1. C-9 genotype is utilized in 2nd phase of project for evaluating performance under red & laterite soils (PIE02002SI)
15

PPS 3559: Testing of carbon capturing potential of mulberry in different locations

April 2015 - March 2018

  1. Assessment of carbon capturing potential of mulberry in varying eco-geographic conditions.
  1. The order of annual carbon credit (x 10-9 Gt/ ha) in study locations were:
    Kalimpong (0.36) >Jorhat (0.31) >Koraput (0.21) > Ranchi (0.15).
  2. Altered farming practice “moderate tillage with grass cover” promises to enhance carbon capturing potential of mulberry substantially on long term basis through continuous improvement of SOCS
  1. Altered farming practice, “moderate tillage with grass cover” promises to enhance SOCS potential in mulberry crop.
16

PPA 3499: Evaluation of field level performance of Vishala mulberry variety in different locations under irrigated conditions in West Bengal

April 2013 - March 2018

  1. To find out the potentiality of Vishala mulberry variety under irrigated conditions of West Bengal.
  2. Field evaluation of Vishala in WB under irrigated conditions
  1. Vishala mulberry variety exhibited 10.98% higher leaf yield potentiality than check, S1635 in irrigated conditions of WB.  
  2. No significant differences were observed with regard to leaf quality.
  1. Vishala variety was considered as National Check for AICEM-III only.
  2. Since then replaced with C-2038
17 

PPS 3598: Arsenic contamination in mulberry sericulture of Bengal Plain and its alleviation through application of zinc in soil

Nov 2016 - Aug 2018 (Pre-mature in May-18 )

  1. To investigate extent of arsenic load in water-soil-mulberry-silkworm continuum mulberry vegetation.
  2. To formulate dose of zinc for alleviating arsenic pollution.
  3. To validate efficacy of laboratory findings
  1. Total arsenic (As) content in water samples (0.0075- 0.669mg/l); soils (2.3-21.1mg/kg); mulberry leaf (5.2-34mg/kg); larvae (0.004-0.04mg/g), which are very low and did not cause any harmful effects on both mulberry and silkworms.
  2. Order of arsenic-abundance in irrigation water-soil-plant-silkworm continuum was observed as follows: Water < Soil (available) <Silkworm (V instar larva) < Mulberry leaf
  1. Lower Arsenic contamination in mulberry and silkworm did not cause any hazardous effects in sericulture. (upto 21.13 mg Avl.As kg-1). 
  2. Information shared with the DoS
18 

PIC 3554: Candidate gene based molecular marker(s) for screening promising recombinants in mulberry

Jan 2016 - Dec 2018

  1. To develop candidate gene based molecular markers of nitrate reductase (NRA) and chalcone synthase in mulberry
  1. Eight high yielding mulberry progenies were identified with high nitrate reductase activity (8.79 -16.45 ΔAg-1FWh-1), better leaf quality & propagation traits as compared to V1
  1. Eight high yielding mulberry progenies with high NRA and its association with better leaf quality & propagation traits is being utilized in the collaborative project with SBRL (PRP08002MI)
19 

PPF 3585: Application of growing degree days as a model driver for developing mulberry yield weather model

Oct 2016 - Dec 2018

  1. To prepare a model driven by GDD towards forecasting growth and yield   mulberry leaves under the spell of daily accumulated heat & basic  weather factors
  1. Developed growing degree days based robust models having highest R2 (>0.88) & lowest RMSE values to predict/forecast mulberry leaf yield of S-1635 under different spacing’s:
    3' x 3' spacing Y= e(6.483+(-361.802/GDD))
  2. 2' x 2' spacing Y= e(5.984+(-369.454/GDD))
  1. Best fit robust models would be utilized for mulberry yield estimates based on GDD values
20 

PPA 3588: Evaluation of low-cost drip fertigation systems on yield and quality of mulberry leaves

Oct 2016 - March 2019

  1. To evaluate the comparative performance of drip tape (DTF) & drum kit (DKF) fertigation system on yield & quality of mulberry.
  2. To optimize fertigation schedule for higher leaf productivity.
  3. To evaluate the fertigation systems on WUE & NUE.
  4. To compute economics of fertigation systems.
  1. 75% Recommended dose of fertilizer (RDF) through drum kit & drip tape fertigation systems increased leaf yield by ~30% t/ha, total leaf soluble protein (~56%), water use efficiency (~67%) & nutrient use efficiency (~66%) over control.                                                  
  2. Both drumkit drip fertigation and drip tape fertigation systems were found to be equally performing with respect to yield and quality in mulberry
  1. 75% RDF with drip fertigation is recommended for mulberry for further popularization under ToT programmes
21

PPA3560: Studies on high bush and tree type mulberry plantation under rainfed condition of Odisha

April 2014 - March 2019

  1. To develop a package of practice for high bush and tree type mulberry plantation to avoid grazing problems in Odisha.
  2. To obtain optimum leaf yield, check cattle menace and curtail maintenance
  1. 8ft x 8ft spacing (tree plantation) was found to be 25-30% more productive in both the mulberry varieties, S1635 & C1730
  1. Wider spacing with tree type plantations (8ft x 8ft spacing) was 25-30% more productive.
  2. The outcome is included in the ECP/TOT programs
22

PIB 3505: Development of Drought Tolerant Mulberry variety for Rainfed Sericulture

Jan 2014 - Dec 2019

  1. Development of drought tolerant mulberry variety
  1. 2190 hybrid seedlings were preliminary assessed under PRT from ten crosses.
  2. Thirty superior genotypes were selected based on Drought tolerant indices and evaluation for two years under rainfed PYT.
  3. PYD 08, 01, 21, 04 & 07 were identified as high yielding genotypes (1.72 -1.96 kg/plant/yr (~>6% over C-2038 & drought tolerant variety C1730).
  1. Five genotypes viz., PYD 08, 01, 21, 04 & 07) were identified as high yielding and drought tolerant over C-2038 & C-1730.
23

PPS 3600: Soil health card preparation for mulberry growing soils in Eastern and North Eastern India

Nov 2016 - Oct 2019

  1. To analyze the soil parameters of Mulberry growing soils for preparation and distribution of soil health card to the sericulture farmers in E & NE India
  1. Soil samples were analyzed & SHCs were digitized and distributed (18076) in E & NE states against the target of 18000.
  2. Necessary remedial measures were suggested in SHC for improving soil health and fertility.
  1. 18076 sericulture farmers of E & NE India benefited by SHCs on soil nutrient status and remedial measures.
  2.  SHCs data uploaded in SHC portal of GoI
24

PPA 3622: Popularization of high bush mulberry plantation technology in Majuli, river island of the Brahmaputra, Assam

Sept 2017 -
Aug 2020
(midterm close
in July 2019)

  1. To assess growth characters, leaf yield of high bush mulberry plantations and cocoon productivity.
  2. Assessment of mulberry pest and diseases
  3. To popularize high bush plantations in river island Majuli, Assam
  1. Single mulberry garden (S1635) with farmer is available. The envisaged variety was C2028.
  2. The data collected might not be useful to draw any logical conclusions and the project was discontinued with due approval from RAC.
  1. Popularizing C-2028, a flood tolerant variety at Majuli Island through ToT programmes
25

PPA 3613: Studies on drum kit irrigation with Hydrogel on yield and water use efficiency of mulberry

Dec 2017 - Nov 2019

  1. To study the water use efficiency by treatment of Hydrogel in mulberry plantation along with Drum kit drip irrigation.
  2. To study the growth and yield performance of mulberry by application of Hydrogels.
  3. To study the cost of production of mulberry leaf by application of Hydrogel & Drum kit irrigation
  1. Alternate day irrigation with DKT & hydrogel application resulted in economical (Rs. 1.37) and effective leaf yield [4.78 MT/ha/crop; ~30% over control (3.20 MT/ha/crop)].
  2. The recommendation could be implemented for rainfed areas.
  1. Popularization through ECP/ToT programmes in the state are essential for effective utilization
III. PEST & DISEASE MANAGEMENT (of host plants & silkworms)
1

CSS 2107: Development of forecasting and forewarning system of mulberry diseases

Oct 2005 - Mar 2012

  1. Development of long term and broad-spectrum database on diseases affecting the production of mulberry
  2. Development of database on meteorological factors and identification of critical weather factors conducive for incidence, intensification and spread of diseases, and to establish disease -weather relationship.
  1. Developed month-wise disease calendar based on meteorological conditions at 14 locations of E & NE India.
  2. Identified Acremonium roseogriseum, a new endophytic fungus (HCIO-50149) collected from Aizawl.
  3. Installation of Automatic weather station (AWS) at RSRS-Kalimpong; RSRS-Koraput & CSRTI-BHP.
  4. Developed disease forecasting model for BLS (Bacterial leaf spot) disease with R2 51% for Murshidabad district
  5. Y = 18.624 – 0.715 X1 + 0.850 X2 – 0.336 X3 + 0.360 X4 + 0.353 X5 + E
    (where, X1 = Max. temp.; X2 = Min. temp.; X3 = Max. RH; X4 = Min. RH; X5 = Rainfall & E= Random error)
  1. Utilized for developing Month-wise disease forewarning calendar for management of mulberry diseases at field level
2

PIG 3441: Development, validation and utilization of SCAR marker(s) for powdery mildew (Phyllactinia corylea) resistance in mulberry. [Collaborative with CCMB, Hyderabad]

Nov 2009 - Oct 2012

  1. Development of SCAR marker(s) linked to resistance response to powdery mildew.
  2. Validtion of the developed SCAR marker(s) for their stability/inheritance using powdery mildew specific mapping progenies.
  3. Testing the validated SCAR marker(s) fir their potential utility as efficient selection markers in MAS approach based disease resistance breeding
  1. Development /establishment of an important genetic resource of mulberry in the form of three mapping populations for dissecting out the inheritance of PM resistance.
  2. Elucidated PM resistance control by multiple QTLs with the recessive alleles and additive gene action.
  3. Developed 2-3 partially validated SCAR and SSR marker(s) for possible use as molecular tags for identification of PM reaction.
  4. Identification of a few transgressive progenies from the S-1 x Vietnam cross having better yield potential than S-1 and resistance to PM. Moreover, all such lines were positive for the putative SCAR markers for PM resistance
  1. Utilized markers for the development of PM resistant variety (C-1360; Ganga) in mulberry
3

ARE 3464: Biology and feeding efficacy studies of Scymnus pallidicolli (Mulsant) for the eco-friendly management of pink mealy bug, Maconellicoccus hirsutus

Oct  2011 - Sept 2013

  1. To study life cycle, longevity and fecundity of native predator on pink mealy bug, Maconellicoccus hirsutus (Green).
  2. To find out feeding efficacy of predator on life stages of mealy bug
  1. Predator life cycle was completed in 23 days (4 days pre-mating, 31 days mating, 21 days post mating period, on 5th /6th day after emergence for first mating).
  2. The pre-oviposition and oviposition period is 10.45 and 32.35, respectively.
  3. Male predator consumed 1612.4 eggs, 211 nymphs and 32 adults; while female predator consumed 1672.4 eggs, 231 nymphs and 42 adults of mealy bug during its life period
  1. The predator (Scymnus pallidicolli ) being utilized for eco-friendly management of mealy bug in mulberry of E & NE states
4

DBT funded:
Development of DNA marker based genetic linkage map of mulberry and QTL analysis for agro-nomically important Planta traits. [Collaborative with CCMB, Hyderabad]

Mar 2011 - Feb 2014

  1. Recovery of the sequence information of 75-mulberry specific markers 
  2. Development of ~500 mulberry specific SSRs
  3. Construction of linkage map using Mysore local x V-1 mapping population
  4. Phenotyping mapping population
  5. Linkage analysis to identify major QTLs
  6. Crossing progeny for trait refinement
  1. Identified high yielding promising lines (127 nos.) as resource material in breeding utilization and for direct field application.
  2. Identification of major QTLs for important agronomic/disease resistant traits would enable to establish the MAS based utility breeding in mulberry
  1. Identified promising progenies would be utilized for direct field utilization through MAS mulberry breeding programmes
5

PRE 3508: Studies on standardization of mass multiplication and field efficacy of Scymnus pallidicolli (Mulsant) for eco-friendly management of Tukra

April 2014 - March 2016

  1. To study intrinsic rate of increase of native predator in ambient conditions
  2. To standardize mass multiplication techniques
  3. To determine optimum number of predators released per unit area
  1. Mass multiplication of Scymnus pallidicolli was standardized.
  2. The life cycle of predator under ambient conditions completed in 27-29 days
  1. The predator (Scymnus pallidicolli ) is being utilized for eco-friendly management of mealy bug in mulberry of E & NE states
6

PRE 3511: Studies on predatory efficacy of coccinellid predator, Scymnus posticalis Sicard for management of white fly on mulberry

April 2014 -March 2016

  1. To evolve standardization of mass multiplication technique of the predator, Scymnus posticalis Sicard;
  2. To evaluate predatory efficiency of Scymnus posticalis Sicard against whitefly, Dialeuropora decempuncta on mulberry
  1. Life cycle of predator was 23-31 days
  2. Mass culture of Scymnus posticalis Sicard failed at RSRS-Jorhat due to predators’ prolonged life cycle & low fecundity
  3. Results were also observed to be inconsistent.
 
7

PPS 3504: Study of root rot disease of mulberry in the Gangetic plains of West Bengal and development of its control measures

April 2014 - March 2017

  1. Preparation of data base on severity of root rot of mulberry in plains of West Bengal.
  2. Development and popularization of plant root protection methods
  1. The chemical (Carbendazim 12%+ Mancozeb 63% @ 0.2%) found to be most effective for management of root rot disease.
  1. Field validation of developed combination along with rot fix is under the project (PRP 02003SI)
8

CSS2107: Forewarning of mulberry diseases of eastern and North Eastern India

April 2012 - March 2017

  1. Collection of disease incidence  and meteorological data
  2. Publicity forewarning system
  3. Fine tuning of existing disease forecasting models
  1. Broad-ended mulberry disease data base of Eastern and North Eastern India
  2. Existing models were fine tuned and models developed when severity of disease is > ETL
  1. Disease specific forewarning models/calendars were developed for the management of mulberry diseases
9

ARP 3516: Studies on synbiotic (Combination of probiotic and pre biotic) induction for control of common disease of silkworm, Bombyx mori L

Oct 2014 - Sept 2016

  1. Application of synbiotics for eco-friendly silkworm disease management
  2. Strengthen immunity of silkworm to resist microbial pathogens for good cocoon yields
  1. Efficacies of 7 synbiotics were evaluated in silkworm. No synbiotic combination was effective in silkworm
  2. The strain specificity of probiotic microbes also plays a significant role in resulting the beneficial effects on the host
  1. Probiotic strains specific to silkworm could not be achieved
10

PPE 3517: Population interaction of pest and natural enemies in mulberry   ecosystem

Aug 2014 - July 2017

  1. To study interactions of host plant, major mulberry pests and natural enemies and correlation with abiotic factors.
  2. Studies on biological attributes of the parasitoids
  1. Pest incidence in mulberry is directly correlated to weather/abiotic factors & natural enemies (predators & parasitoids).
  2. Naturally prevalent parasitoids are capable enough to control the pest, so that equilibrium balance is maintained in the mulberry eco-system.
  1. Culture of natural enemies and their release holds an important role in diminishing the pest incidence at field level.
11

PRE 3533: Incidence of whitefly in Mulberry germplasm accessions

Mar 2015 - Feb 2018

  1. To observe incidence of whitefly in mulberry germplasm for identifying putative sources of resistance and susceptibility.
  2. To evaluate germplasm accessions for whitefly resistance using physiological & biochemical indices
  1. 154 mulberry accessions were evaluated for whitefly incidence under natural conditions. Ten accessions were found to be relatively resistant (1-4 flies/leaf) to whitefly.
  1. Ten mulberry accessions identified for tolerance to whitefly are being explored further
12

ARP 3522: Isolation, cloning and characterization of antibacterial protein(s) from Bombyx mori L.  (Collab. with SBRL, Kodathi, Bangalore)

May 2015 - June 2018

  1. To isolate and characterize  bacterial strains and study pathogenicity.
  2. To isolate, purify and characterize antibacterial protein fractions elicited against bacterial strain.
  3. Molecular cloning using cDNA cloning for mass production of ABP
  1. Apolipophorin-III was successfully cloned and expressed in Pichia pastoris. Recombinant lipoprotein of ~20kDa & ~33kDa exhibited antibacterial activity against pathogenic bacteria
  1. Antimicrobial gene, Apol-III was expressed in P. pastoris for expression of ~20kDa & ~33kDa ABPs.
13

PIB 3548: Evaluation of bacterial leaf spot resistant improved progenies of mulberry for field utilization

Jan 2016 - Dec 2018

  1. Evaluation of bacterial leaf spot (BLS) resistant improved lines for foliage biomass and associated agronomic traits. 
  2. Silkworm bioassay
  3. Development of advanced breeding generation (F2) through sib-mating of promising lines (F1).
  4. Evaluation of BLS resistance in F2 by SSRs for MAS
  1. BLS resistant genotype, C-7 exhibited significantly higher resistance response (61-72%) than commercial /pipeline cultivars viz., S-1, C-2028, S-1635 & C-2038
  2. Annual leaf biomass potential of C-7 was significantly higher (10458-11558 kg/ha/crop) than C-2038
  3. Two allelic bands of SSRs viz., MM17 (182bp) and NGS 263 (286bp) showed very strong correlation with phenotypic disease reaction over three generations.
  1. Identified better yielding resistant genotype (C-7) is considered in FYT project as well as OST programme.
14

PRE 3589: Assessment of designed antimicrobial peptides for mulberry protection against brown leaf spot and root rot: a biotechnological approach

Oct 2016 - Sept 2019

  1. Assessment of disease protection potential of synthetic antimicrobial peptides (AMPs)  against Myrothecium leaf spot (MLS) and Fusarium root root (FRR) of mulberry Assessment of disease protection potential of selected AMPs against MLS and FRR ex-vivo
  2. Determination of disease suppression ability of selected AMPs using quantitative PCR
  1. PRE-2 exhibited maximum conidial germination and  mycelia growth suppression abilities against M. roridum (MIC50: 23.1µM) and F. solani (MIC50:14.6µM)
  2. 75µM PRE-2 suppressed MLS disease severity by 41%
  3. 100µM PRE-2 suppressed FRR by 55% symptoms and restored 48% leaves and 67% leaf area
  4. PRE-2 application inhibited fungal multiplication (qPCR; MLS: 1.7-folds; FRR: 5.5-folds)
  1. Novel process of Anti Fungal Peptide (AFP2) mediated suppression of Fusarium dry root rot  (Fusarium solani) & Myrothecium leaf spot (Myrothecium  roridum) in  mulberry
15

ARP 3590: Studies on the efficacy of phototrophic bacterial extracts as feed supplements for management of diseases in silkworm Bombyx mori L

Oct 2016 - Sept 2019

  1. To screen the efficacy of phototrophic bacterial extracts as feed supplements for disease management in silkworm.
  2. To prepare metabolite profiling of silkworm when fed on normal and phototrophic bacterial extract enriched diet
  1. Three phototrophic bacteria isolated were identified as Marichromatium spp., Rhodopseudomonas spp. and Rhodobacter spp. None of the phototrophic bacterial feed supplements exerted toxic on rearing and reeling traits
  2. The survivability increased by 16% in batches fed with Rhodopseudomonas sp. feed supplementation against Staphylococcus sp. No significant differences were observed in metabolites of silkworm fed on normal and phototrophic bacterial fortified diets
  1. Utility of FS is very much limited as it is not effective against other pathogens. 
IV. SOCIAL SCIENCE
1.

DST funded project: Improvement of mulberry seed cocoon production at farmers’ level with special reference to Bivoltine seed cocoon in West Bengal.

[Collaboration with DoT (Seri), West Bengal and NSSO, Bangalore]

Nov 2011 - Oct 2012

  1. Stabilization and improvement of Bivoltine and Multivoltine seed cocoon crops especially during adverse crop of West Bengal & establishment of model seed rearers
  1. Average annual income of an adopted farmer was Rs. 64,129/-, which is 38% more than the non-adopted farmers.
  2. Improvement in seed cocoon production             (7 kg/100 dfls i.e. 28%) in spite of varied climatic conditions
  3. A glimpse of light for expanding Bivoltine Sericulture in West Bengal has been found by advocating Bivoltine seed cocoon production throughout the year at Kiriteswari village with an average cocoon yield of 33.5 kg/100 dfls
  4. Up-liftment of socio-economic condition (additional income) was observed ~25% at adopted seed rearers (16-38%) through enhancement of quality cocoon production
  1. Served as initiation for bivoltine seed production in West Bengal 
2. 

 MOE 3459: Yield gap in mulberry sericulture– A study in North-Eastern region of India

Oct 2011 - April 2014

  1. Study the gap of yield between research station and farmers’ field
  1. Socio-organization status gap was significant between demo & farmers’ plots
  2. Mulberry cultivation and silkworm rearing practices gaps were due to lack of technological knowledge and infrastructure.
  3. Lack of knowledge on chawki rearing package (100%) was maximum, partial adoption of black boxing technology was high among the farmers.
  1. Information shared with DoS-Assam/ Extension workers to focus further for knowledge transformation ways in order to minimize the gaps and improve crop yield in the region
3.

MTS 3599: Study on mulberry sericulture production in West Bengal: a statistical approach

Nov 2016 - April 2018

  1. To analyze the trends in mulberry cocoon production in West Bengal.
  2. To estimate costs and returns of mulberry cocoon production.
  3.  To assess resource use pattern and resource use efficiency of sericulture farmers
  1. Mulberry sericulture scenario in West Bengal showed cubic trend with negative CGR for mulberry acreage (-1.38) and exponential trend with positive CGR for cocoon (2.35) & raw silk (3.72) since 1989 to 2018
  2.  Return per rupee investment for cocoon production was higher with small farmers (1:1.58) as compared to marginal famers (1:1.47).
  3. 70% farmers were resource use-efficient with regard to economic efficiency
  1. Outcome of study has been shared with DoS, West Bengal for developmental project in sericulture.
4.

MOT 3601: Skill gap analysis and capacity development  of  sericulture  extension  workers  and farmers  in  traditional  and  non-traditional states

Nov 2016 - April 2018

  1. To document job profile of extension workers in sericulture and their engagement.
  2. To identify skill requirements for extension and farmers.
  3. To determine skill gap of extension and farmers.
  4. To design CBTs for skill requirements of extension workers and farmers
  1. Skill gap analysis in Eastern zone indicated lower skill status with farmers (~11%) and ~80% for extension workers.
  2. Technology demonstrations with regard to essential skills for mulberry cultivation & silkworm rearing ranked first.
  3. Training manual for skill upgradation (CBT) was prepared & is being utilized for skill upgradation programmes
  1. Outcome of the study is being utilized as a bench for training programs on skill upgradation in the institute.
5. 

MOE 3604: Yield gap analysis in mulberry leaf and cocoon production - A study in Eastern ghat highland zones of Odisha

Dec 2016 - Nov 2018

  1. To assess the magnitude of ‘Yield Gap’ in respect of mulberry leaf and cocoon productivity at farmers level.
  2. To examine the nature of variation in ‘Yield Gap across different socio-economic strata of the sericultural farmers.
  3.  To identify factors influencing for ‘Yield Gap’.
  4. To suggest policy measures for reducing the gap
  1. The yield gap recorded in the annual leaf productivity is ~13% yield gap (demo plots) & 79 % (farmers) and ~28% yield gap (DP) & 71% (farmers) average cocoon productivity in respect of Research institute. 
  2. The constraints enlisted as major factors for the observed yield gap include poor socio-economic status, low operational land holding, farmer’s perception on silkworm rearing (seed crop), low adoption of technologies, poor extension & training activities.
  3. The yield gap from Research Institute to Farmers Plot is huge and it could be reduced through adoption of sericultural technologies; intensive extension & training activities by the concerned departments.
  1. The yield gap from Research Institute to Farmers Plot is huge and it could be reduced through adoption of sericultural technologies; intensive extension & training activities by the concerned departments.