(Mrs.) Rita Banerjee,
Scientist – D
hasten the targeted improvement of mulberry by harnessing
molecular marker technologies.
of agronomically important traits of specific mapping progenies
to support construction of linkage map and dissecting important
facilitate rapid screening and assessment of biotic and abiotic
responsive important traits of mulberry utilizing various tissue
source of resistance to Powdery mildew disease of mulberry
through conventional symptomatic screening under natural and
artificial epiphytotics. Identified a number of putative DNA
tags /segments associated with powdery mildew resistance
(collaborative study with CCMB, Hyderabad).
and in planta screened
a mapping population (-150nos) derived from the cross of two
well-adopted and widely cultivated varieties of mulberry (Mysore local
protocol for direct shoot initials development from leaf disc
explants of commercial cultivars of mulberry (S-1).
two major sources of bacterial leaf spot resistance(BLS) through
in-depth screening trials, establishment of BLS specific mapping
progeny and analyzed
the genetics of the resistant trait
Incubator with shaker
Speed Refrigerated Centrifuges
Binocular Compound Microscope
Gel Electrophoresis systems
bath with standard heating block
ONGOING PROJECT (02):
DBT supported project 1
Development, validation and utilization of SCAR marker(s) for
powdery mildew (Phyllactinia corylea)
resistance in mulberry
(Nov. 2009–Oct. 2012) (Sanction No. BT/PR11675/PBD/19/196/2008
dated 12.10.2009; in collaboration with CCMB- Hyderabad)
of SCAR marker(s) linked to resistance response to powdery
of the developed SCAR marker(s) for their stability/inheritance
using powdery mildew specific mapping progenies.
the validated SCAR marker(s) for their potential utility as
efficient selection markers in MAS approach
based disease resistance breeding.
Specific objectives of the Institute:
of powdery mildew specific mapping progeny.
of progeny by transfer of resistant trait to improved strains
for MAS based breeding approach.
of mapping population for powdery mildew resistance and other
associated parameters for SCAR
DBT supported project 2
Development of DNA marker based genetic linkage map of mulberry
and QTL analysis for agronomically
important planta traits (March
(Sanction no. BT/PR11872/PBD/19/200/2009 dated 16.03.2011; in
collaboration with CCMB- Hyderabad)
of mulberry specific SSR markers for genetic studies.
of reasonably dense molecular linkage map using the ‘Mysore local
x V-1’ mapping population and different types of molecular
of mapping population for various agronomical traits including
response to some important foliar diseases.
analysis to identify major QTLs linked to some important traits
Specific objectives of the Institute
phenotyping of the mapping population for various agronomical
traits and responses of important foliar
of some promising progeny plants for possible trait refinement
and/or mapping validation.
BPP(PS)-001: Improvement of Mulberry through in vitro
mutagenesis and somaclonal variation
Duration - 18 months (July 2011- Dec. 2012)
develop putative mutants by employing chemical mutagens in
mulberry genotypes S1635 and C2038
develop somaclonal variants of mulberry genotypes S1635 and
supported project: Mulberry
genome characterization: DNA profiling for ascertaining genetic
diversity and construction of framework linkage map (2003
–2007). (A collaborative
project with CSR&TI, Mysore and
the clonal representation of a mapping population derived from
the cross of Mysore local
framework linkage map of mulberry utilizing RAPD, AFLP and
in-house developed microsat markers at CCMB
many promising progeny plants with desired improved features
based on in planta characterization.
supported project: Identification
of DNA markers associated with disease and pest resistance in
mulberry (Morus spp.)
( 2005-2008) (DBT funded project in net working mode with
CCMB, Hyderabad, CSR&TI, Mysore, CSGRC, Hosur and KSSR&DI,
144 potential core germplasm resources (exotic 61 and indigenous
83) for source of resistance to Phyllactinia
natural mildew infection and under artificial epiphytotics.
powdery mildew responsive resistant and susceptible accessions.
DNA tags/segments for powdery mildew resistance at CCMB.
Funded Project: PIT
3359-Development of high frequency regeneration protocol from
leaf disc explants in mulberry (2006-2009)
of high frequency regeneration protocol of plantlets from leaf
disc explants of mulberry
optimum shoot regeneration from axillary bud explants was
observed at 2.0 and 1.0 mg/L 6-BAP and also in 0.2 and 0.1 mg/L
TDZ concentrations on MS medium in S-1 and S1635, respectively.
formed roots on MS medium containing NAA (0.5 - 0.75 mg/L) in 3
weeks of culture
Funded Project: PIE-3319:
Screening of germplasm and raising of progeny towards
development of disease resistant mulberry against bacterial leaf
spot (May 2005 –
two major sources of resistance (M. rotundiloba & M.
three susceptible (KPG-1, Nagaland local and Bogura-4)
continuous variation for bacterial leaf spot (BLS )
responsiveness among the progenies suggest
quantitative nature of resistance in mulberry.
estimates (both broad and narrow senses) indicate polygenic
nature of BLS resistance with additive gene effect.